pca cell lines pnt1a Search Results


pca cell lines pnt1a  (ATCC)
99
ATCC pca cell lines pnt1a
Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in <t>PNT1A,</t> LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .
Pca Cell Lines Pnt1a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in PNT1A, LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Elongation and ROS-Mediated Apoptosis in Prostate Cancer Cells under Therapy with Apalutamide and Complex I Inhibitor

doi: 10.3390/ijms25136939

Figure Lengend Snippet: Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in PNT1A, LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .

Article Snippet: PCa cell lines PNT1A (prostate epithelial cells), LNCaP (androgen-dependent) and PC-3 (androgen-independent) were all purchased from ATCC.

Techniques: Western Blot, Expressing

Assessment of apoptosis in PCa cells. ( A ) Representative flow cytometry dot plots of cells stained with Annexin V/PI. PCa cell lines PNT1A, LNCaP, C4-2 and PC-3 were treated for 72 h with DMSO, ARN, IACS and Combo. The sum of Annexin V (+)/PI (−) and Annexin V (+)/PI (+) cells in the lower right and upper right corner was used to quantify apoptotic cells. ( B ) Bar plots represent three independent experiments of measurements of apoptotic cells for PNT1A, LNCaP, C4-2 and PC-3. Bar plots are shown as means ± SEM. * p = 0.0179; ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Elongation and ROS-Mediated Apoptosis in Prostate Cancer Cells under Therapy with Apalutamide and Complex I Inhibitor

doi: 10.3390/ijms25136939

Figure Lengend Snippet: Assessment of apoptosis in PCa cells. ( A ) Representative flow cytometry dot plots of cells stained with Annexin V/PI. PCa cell lines PNT1A, LNCaP, C4-2 and PC-3 were treated for 72 h with DMSO, ARN, IACS and Combo. The sum of Annexin V (+)/PI (−) and Annexin V (+)/PI (+) cells in the lower right and upper right corner was used to quantify apoptotic cells. ( B ) Bar plots represent three independent experiments of measurements of apoptotic cells for PNT1A, LNCaP, C4-2 and PC-3. Bar plots are shown as means ± SEM. * p = 0.0179; ** p < 0.01.

Article Snippet: PCa cell lines PNT1A (prostate epithelial cells), LNCaP (androgen-dependent) and PC-3 (androgen-independent) were all purchased from ATCC.

Techniques: Flow Cytometry, Staining